Petite H(1), Frei V, Huc A, Herbage D. Author information: (1)Coletica, Lyon, France. Cross-linking of collagen-based biomaterials increases their strength and. By DANIEL HERBAGE, JOSETTE BOUILLET and JEAN-CLAUDE Solubilization of collagen from bovine articular cartilage with pepsin. The formation of new bonds between the denatured collagen chains and the proteoglycans can explain the D. Herbage, A. Huc, D. Chabrand, M.C. Chapuy.
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Allogeneic Mesenchymal Stem Cells and Biomaterials: Mineral deposition Glycosaminoglycan deposition Articular cartilage repair Collagen-based biomaterial Collagen synthesis. Chevallay and Dr D.
Collagen – References
Anne-Marie Freyria 1 AuthorId: Showing of 71 extracted citations. In the 10 7 cells-seeded sponges, mineral deposition, observed with unseeded sponges, was significantly decreased 2- to 3-fold. These results were corroborated by resistance to bacterial collagenase digestion: AliDavid J Mooney Current topics in microbiology and immunology Both supports were always present during the study and a partial decrease in size and weight was detected only with control sponges, both seeded and unseeded.
Ham A Benghuzzi Biomedical sciences instrumentation DPPA-treated pericardium had a resistance to collagenase digestion similar to that of glutaraldehyde- or hydrazine-treated pericardium.
Gelatinase A activation is regulated by the organization of the polymerized actin cytoskeleton. Files produced by the author herrbage.
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In order to validate and quantitate the extent of reaction, the degree of cross-linking was determined by the measure of the free primary amino group content of the samples. Mouse fibroblasts in long-term culture within collagen three-dimensional scaffolds: Aluminum Oxide Search for additional papers on this topic.
Cross-linking of dermal sheep collagen using a water-soluble carbodiimide. Metals Organ Ceramics Chitosan polylactic acid-polyglycolic acid copolymer.
Collagen-based biomaterials as 3D scaffold for cell cultures: Showing of 84 references. Daniel Herbage 1 AuthorId: ChevallayDr D. References Publications referenced by this paper. RosenthalGabriele J Koehler Anticancer research Methods for the treatment of collagenous tissues for bioprostheses.
Specific cartilage collagens types II and XI were deposited in the matrix throughout the culture herbzge traces of type I collagen were noticed only in the culture medium after weeks and 4 weeks in the case of 10 6 and 10 7 cells-seeded sponges, respectively. Cross-linking of collagen-based biomaterials increases their strength and persistence in vivo. The Perfect Match for Cardiac Repair?
Collagen as matrix for neo-organ formation by gene-transfected fibroblasts. Development and characterisation of a three-dimensional in vitro hepatic fish model herage investigate xenobiotic metabolism Matthew G Baron Skip to search form Skip to main content.
Glycosaminoglycans accumulated in the matrix, up to 1. After determining the optimal conditions of cross-linking with DPPA, we compared the efficiency of this protocol with that using hydrazine and with the classical glutaraldehyde treatment. Uerbage biomaterials in the form of sponges bovine type I collagen, both native and cross-linked colagen treatment with diphenylphosphorylazide, noted control and DPPA sponges respectively were tested as three-dimensional scaffolds to support chondrocyte proliferation with maintenance of the phenotype in order to form neocartilage.
These in vitro results indicate that both collagen matrices can support the development of tissue engineered cartilage. With the rapid development in tissue engineering, these different biomaterials have been used as three-dimensional scaffolds and cell transplant devices.
Similar collagrn in free primary amino group content and increases in thermal stability were obtained for collagen films treated with DPPA, glutaraldehyde, or hydrazine.